Tóm tắt:

This study was conducted to evaluate the ability to eliminate iCasp9-IL15 Chimeric Antigen Receptor-T (CAR-T) cells using the photosensitizers AP20187 in vitro. Peripheral blood mononuclear cells (PBMC) were activated with Dynabeads Human T-Activator CD3/CD28 and IL-2 to optimize the concentration of the antibiotic blasticidin for screening iCasp9-IL15 CAR-T cells. iCasp9-IL15 CAR-T cells after proliferation with 1D2 artificial antigen-presenting cells, were screened using blasticidin. Subsequently, the iCasp9 suicide gene of these cells was activated at various concentrations of AP20187 photosensitizer.