Tóm tắt:

Pueraria mirifica is a popular medicinal plant and is rich in phytoestrogens, especially isoflavonoids. The chalcone isomerase (CHI) gene is well-known as a key factor in biosynthetic pathways such as flavonoid, isoflavonoid, and anthocyanin biosynthesis. Among four CHI subfamilies, group IV remained largely uncharacterized in P. mirifica. By isolation and gene cloning, the CHI4A gene was amplified and sequenced. A dataset that included CHI4A genes from isolation, transcriptome, and Glycine max CHI4A was constructed for analysis.