Tóm tắt:

The majority of autoimmune and allergic diseases are associated with abnormal expression of interleukin (IL)-33, a member of the IL-1 family of cytokines, that function dually as a proinflammatory cytokine and a transcriptional factor. We created an IL-33 inhibitor called "IL-33 Trap Fc" constructed by fusion of an Fc fragment of human immunoglobulin G1 and two distinct extracellular part receptors involved in interacting with IL-33, IL-1 receptors accessory protein, and IL-33 receptor. IL-33 Trap Fc was expressed by two systems, mammalian HEK293 cells and Pichia pastoris yeast. These data collectively suggested that IL-33 Trap Fc potently blocks IL-33 in vitro and in vivo, which may be a novel therapeutic strategy for IL-33-mediated allergic diseases.