This research aimed to explore the anti-inflammatory effects of n-hexane, ethyl acetate (EtOAc), and n-butanol extracts from the leaves of Ardisia silvestris Pitard, both in vitro and in vivo, specifically focusing on the EtOAc extract. The in vitro assessment of anti-inflammatory activity involved measuring the inhibition of nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophage cells. The findings revealed that the EtOAc extract exhibited the highest potency, with an IC50 value of 52.10 ± 2.07 µg/mL, effectively inhibiting NO production without causing cellular damage. In vivo, the EtOAc extract’s anti-inflammatory properties were evaluated using carrageenan-induced paw edema in rats and the cotton pellet-induced granuloma test in mice. At both tested dosages, the EtOAc extract significantly reduced paw edema induced by carrageenan in rats. Additionally, it effectively inhibited exudate and granuloma formation following cotton pellet implantation at both doses, indicating its anti-inflammatory activity during the subacute phase of inflammation. Overall, these results strongly suggest that the ethyl acetate extract of Ardisia silvestris Pitard possesses significant anti-inflammatory properties and may be beneficial in traditionally treating various inflammatory conditions.
This study aimed to evaluate the prevalence and associated factors of anxiety among women scheduled for gynecological surgery. Conducted as a cross-sectional analysis, data collection involved structured interviews that assessed demographics, clinical history, perceptions of postoperative recovery, comprehension of surgical risks, and levels of social support. Anxiety was measured using the GAD-7 scale, and associations between anxiety severity and related factors were examined through ordinal logistic regression. Among the 300 participants, all reported some degree of anxiety, with 60% experiencing mild anxiety, 30% moderate, and 10% severe. Higher anxiety levels were notably linked to limited social support and lower comprehension of counseling. The high prevalence of anxiety observed underscores the need for comprehensive preoperative communication and robust support systems. These strategies could play a vital role in reducing anxiety, ultimately contributing to improved surgical recovery and overall patient well-being.
The aim of this cross-sectional study was to identify the association between nutritional status and related factors among uncontrolled type 2 diabetic older patients. Variables including demographic information, body mass index, frailty, depression, physical activity daily living, comorbidity, polypharmacy and diabetic characteristics. Nutritional status was defined by Mini Nutritional Assessment Short Form. Data was entried by Redcap and analised by Spss 22.0 verson. A total 148 participants with mean of age was 76 ± 7.47 years old, and 62.2% were female.
Work motivation is crucial for healthcare workers’ performance and the overall effectiveness of the health systems. A well-motivated healthcare workforce can lead to improved patient satisfaction, reduced errors, and a more sustainable health system. This study aimed to describe the work motivation of healthcare workers in Bac Giang province in 2024 and analyze associated factors. A cross-sectional descriptive study was conducted among 253 health workers in Bac Giang province, Viet Yen and Yen Dzung districts using a quantitative approach with structured questionnaire. The main findings revealed that 80.6% of respondents were categorized as having low work motivation, with an average score of 3.66 (SD = 0.37). Significant associations were observed between work motivation and workplace location, years of working in the health field, and night shift frequency.
Health literacy (HL) is a critical factor in the management of type 2 diabetes mellitus (T2DM), yet many patients lack adequate understanding, leading to poor health outcomes. This study assessed HL among patients with T2DM and identified associated factors. This cross-sectional study involved 358 diabetic outpatients who completed a self-reported questionnaire, including the Health Literacy - Short Form (HL-SF12), Diabetes Self-Management Instruments (DSMI-20), and Multi-Dimensional Support Scale (MDSS).
Breastfeeding is a natural and effective way to protect mothers‘ and children‘s health. This research aims to evaluate knowledge of breastfeeding among mothers with their children under 6 months old visiting the E Hospital, a tertiary hospital in Hanoi, Vietnam. A cross-sectional study was conducted using a predesignated, self-administered questionnaire based on the recommendation from the WHO. A special scaling system was employed to calibrate between different types of questions. Data was collected from the survey from 1/2023 to 10/2023 using the platform REDCap and analyzed using the software SPSS®.
Hyperinsulinism Hyperammonemia Syndrome (HI/HA syndrome) is an autosomal dominant disease caused by activating mutations in GLUD1, the gene responsible for encoding the mitochondrial enzyme glutamate dehydrogenase (GDH). This syndrome represents the second most common genetic form of congenital hyperinsulinism in infancy. Children with HI/HA syndrome typically experience hypoglycemic symptoms triggered by fasting or high-protein meals and persistently elevated ammonia levels. We report a case of HI/HA syndrome in a 7-month-old female who presented with cyanosis and hyperammonemia, along with an initial normal glucose level at the time of presentation. About one week after the child’s admission to the hospital, we discovered that hypoglycemia was the cause of her irritability. The hypoglycemic episode was found to occur coincidentally with hyperammonemia. The combination of clinical findings, biochemical markers, and genetic sequencing identifying a GLUD1 pathogenic variant facilitated the correct diagnosis of HI/HA syndrome. As demonstrated by this case, the diagnosis of HI/HA syndrome requires a thorough clinical evaluation, comprehensive biochemical analysis, and genetic testing. With the correct diagnosis, a patient with HI/HA syndrome can receive ongoing monitoring and care with the goal of preventing adverse neurologic sequelae.
We report two unrelated 8-year-old Vietnamese girls diagnosed with Kenny-Caffey Syndrome type 2 (KCS2), each harboring the same heterozygous pathogenic variant in the FAM111A gene (c.1706G>A, p.Arg569His). Case 1 presented initially at age 4 with poor growth and persistent anterior fontanelle. At age 8, she exhibited severe short stature (-4.5 SDS), macrocephaly, small hands, and craniofacial dysmorphisms. Additional findings included persistent fontanelle, hyperopia with amblyopia, cortical thickening of long bones, and asymptomatic hypocalcemia with normal parathyroid hormone (PTH) levels. Brain MRI showed a thin pituitary gland. Case 2 presented with short stature (-4.96 SDS), a history of hypocalcemic seizures, and congenital astigmatism. She had normal facial appearance but showed cortical bone thinning, absent pituitary lobes, and hypocalcemia with inappropriately low PTH levels. Despite calcium supplementation, normocalcemia was achieved only after calcitriol therapy. Growth hormone was later initiated with favorable growth response. Both cases underscore the variable expressivity of KCS2 and highlight the diagnostic value of genetic testing in children with unexplained short stature, skeletal anomalies, and calcium disturbances. The study contributed additional data on NKX6-2 variants in Vietnamese patients.
Vaginal leiomyomas are rare, benign mesenchymal tumors. Since Denys de Leyden identified the first case in 1733, there have been only around 300 reported cases. This case of a surgically treated vaginal leiomyoma highlights the importance of considering this rare diagnosis in reproductive-age women. We provide a review of the relevant literature on diagnosis and management.
Trong nghiên cứu này, hạt nano bạc được tổng hợp xanh hóa với cao chiết cây Cúc leo (Mikania micrantha Kunth) có vai trò là tác nhân khử tiền chất bạc nitrate và polyvinyl pyrrolidone (PVP) là chất ổn định. Các yếu tố ảnh hưởng đến sự hình thành và phát triển của hạt nano bao gồm nồng độ bạc nitrate, nồng độ cao chiết và thời gian phản ứng được khảo sát với mục tiêu tạo ra hạt nano bạc có hình dạng và kích thước mong muốn. Điều kiện thích hợp để tổnghợp nano bạc từ cao chiết cây Cúc leo bao gồm: nồng độ AgNO3 0,030 M, nồng độ cao chiết là 2,50 mg/ml, thời gian phản ứng là 90 phút tại nhiệt độ phòng. Thông qua kết quả quang phổ - tử ngoại khả kiến (UV-Vis), kính hiển vi điện tử truyền qua (TEM), kính hiển vi điện tử truyền qua phân giải cao (HR-TEM), kính hiển vi điện tử quét (SEM), phổ phân tán năng lượng tia X (EDS), tán xạ ánh sáng động (DLS) và thế zeta cho thấy đã tổng hợp thành công hạt nano bạc có dạng cầu, kích thước trung bình khoảng 47,1 nm. Nano bạc được tổng hợp từ cao chiết cây Cúc leo thân thiện với môi trường hứa hẹn sẽ mang lại nhiều tiềm năng ứng dụng trong tương lai.
