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Trở về

Identification of Panax spp. in the Northern Vietnam based on ITS-rDNA sequence analysis

Tác giả: Tran Thi Viet Thanh, Trinh Ngoc Bon, Do Van Truong, Pham Quang Tuyen, Hua Van Luong, Pham Van The, Phan Ke Long
Số trang: P. 633-641
Tên tạp chí: Công nghệ sinh học - Vietnam Journal of Biotechnology
Số phát hành: Vol. 20(4)
Kiểu tài liệu: Tạp chí trong nước
Nơi lưu trữ: 03 Quang Trung
Mã phân loại: 615
Ngôn ngữ: Tiếng Anh
Từ khóa: Ginseng, internal transcribed spacer (ITS), nucleotide sequence,Panax, P. notoginseng, P. stipuleanatu
Tóm tắt:

In order to accurately identify ginseng species (Panax spp.) that grow naturally in some Northern provinces of Vietnam, we collected 30 natural ginseng samples and used nuclear genetic region (ITS-rDNA) for current analysis. The success rate for nuclear genomic region PCR (ITS-rDNA) amplification is 100%. The bidirectional sequence read success rate obtained from the PCR product was 100%, with a nucleotide sequence length of 588 bp. Based on the analysis of ITS-rDNA region results, the samples of ginseng species from Tuyen Quang and Cao Bang provinces have a close relationship with Panax notoginseng (MLBS = 100%), while the samples of ginseng species from Ha Giang and Yen Bai provinces have a close relationship with P. stipuleanatus (MLBS = 99%).