Tóm tắt:

Beta-glucosidase (BGL) is anenzyme involvedin the degradationof cellulose and playsan essential partin many biological processes. Currently, most BGLs applied in the industryare derived from fungi. Exploring novel BGLswith desired propertiesisattractive. The recombinant BGL derived from microorganismssurroundingwhite-rot fungus in Cuc Phuong National Park was successfully expressed in Escherichia coliRosetta 1(denoted asthe GH3S2 gene).The protein GH3S2was purified by an affinity chromatography column using buffer PBS 50 mM (NaCl-free) pH 7, and the enzymewas collected in buffer containing imidazole 300 mM.