Tóm tắt:

This study aimed to assess the effects of simulated microgravity (SMG) on senescent human fibroblasts (HFs). The HFs were treated with 150 µM H2O2 in a culture medium for 90 minutes for senescent induction. The senescent HFs were induced SMG for 72 hours by a Gravity simulator, while the control group was treated in the same CO2 incubator. Cell morphology analysis showed that the SMG condition retrieved the fibroblastic morphology from the senescence. WST1 assay and cell counting indicated that the senescent HFs viability from the SMG group was higher than the control group (OD value of 0.80 ± 0.13 vs. 0.64 ± 0.11, respectively).