57161Purification and characterization of a recombinant beta-glucosidase in Escherichia coli
Beta-glucosidase (BGL) is anenzyme involvedin the degradationof cellulose and playsan essential partin many biological processes. Currently, most BGLs applied in the industryare derived from fungi. Exploring novel BGLswith desired propertiesisattractive. The recombinant BGL derived from microorganismssurroundingwhite-rot fungus in Cuc Phuong National Park was successfully expressed in Escherichia coliRosetta 1(denoted asthe GH3S2 gene).The protein GH3S2was purified by an affinity chromatography column using buffer PBS 50 mM (NaCl-free) pH 7, and the enzymewas collected in buffer containing imidazole 300 mM.
57162Purification and characterization of recombinant nattokinase from Bacillus subtilis R0H1
In this study, recombinant NK from B. subtilis R0H1 was purified using two-step membrane filtration. Results showed 3.2-fold increase in activity and a recovery rate of more than 80%. Molecular weight of NK was approximately 28 kDa and its fibrinolytic degradation capacity was proved according to SDS-PAGE. The optimal pH and temperature of this NK were 8.5 and 55°C, respectively. The enzyme activity was boosted by Mg2+, Ca2+ and obviously inhibited by Co2+, Zn+2, Fe2+, and SDS. The apparent Km and Vmax with fibrin as the substrate were 3.08 mM and 6.7 nmol/min, respectively. The results suggested that membrane filtration is a useful method for purification of recombinant NK from B. subtilis R0H1. Therefore, application of membrane system is proposed to purify NK at the pilot scale. In addition, our findings indicated that recombinant NK produced in B. subtilis R0H1 showed high and stable proteolytic activity in slightly alkaline pH and at high temperature. It also exhibited strong fibrinolytic activity again both substrates: fibrinogen and fibrin.
57163Purpose, meaning, and passion : HBR emotional intelligence series
This collection of articles includes "Finding Meaning at Work, Even When Your Job Is Dull," by Morten Hansen and Dacher Keltner; "What to Do When Your Heart Isn't in Your Work Anymore," by Andy Molinsky; "You Don’t Find Your Purpose--You Build It," by John Coleman; "How to Find Meaning in a Job That Isn't Your True Calling," by Emily Esfahani Smith; "You're Never Done Finding Purpose at Work," by Dan Pontefract; "From Purpose to Impact," by Nick Craig and Scott A. Snook; "Five Questions to Help Your Employees Find Their Inner Purpose," by Kristi Hedges; "How to Make Work More Meaningful for Your Team," by Lewis Garrad and Tomas Chamorro-Premuzic; "The Power of Small Wins," by Teresa M. Amabile and Steven J. Kramer; and "The Founder of TOMS on Reimagining the Company's Mission," by Blake Mycoskie.
57164Put AI to work for you : the revolution is here!
Unlock the power of AI to enhance every aspect of your life with our comprehensive guide. From smart home management and personal health to career growth and social connections, discover innovative strategies to live smarter and achieve your goals. Embrace technology to streamline your daily tasks, improve your well-being, and expand your opportunities for success.
57165Pửt Tày Bắc Cạn
Giới thiệu một loại sinh hoạt văn hóa tín ngưỡng của người Tày ( gọi là Pửt ).
57166Puzzles for programmers and pros
Providing you with the tools to solve several classes of puzzles by handand computer. These include scheduling,strategic, geometric, and probabilistic puzzles. You'll also find a mystery involving codes, bank accounts, and geography that you can solve for the chance to win a prize. The approaches and techniques in this book will help you solve the kind of application puzzles the real world may throw at you.
57167Python : the complete manual
Everything you need to know to get started with Python! From how the language works to coding projects that are easy and fun to follow, you can get up and running building games, controlling hardware and much more!.
57168Python for kids
Week 1: Slithering into Python; Week 2: Building Guessing Games; Week 3: Creating Word Games; Week 4: Stepping Up to Object?]Oriented Programming.
57169Python in a nutshell : 2nd ed.
This book offers Python programmers one place to look when they need help remembering or deciphering the syntax of this open source language and its many powerful but scantily documented modules. This comprehensive reference guide makes it easy to look up the most frequently needed information--not just about the Python language itself, but also the most frequently used parts of the standard library and the most important third-party extensions.
57170Python phrasebook
Python Phrasebook gives you the code phrases you need to quickly and effectively complete your programming projects in Python.