54061Purification and characterization of recombinant nattokinase from Bacillus subtilis R0H1
In this study, recombinant NK from B. subtilis R0H1 was purified using two-step membrane filtration. Results showed 3.2-fold increase in activity and a recovery rate of more than 80%. Molecular weight of NK was approximately 28 kDa and its fibrinolytic degradation capacity was proved according to SDS-PAGE. The optimal pH and temperature of this NK were 8.5 and 55°C, respectively. The enzyme activity was boosted by Mg2+, Ca2+ and obviously inhibited by Co2+, Zn+2, Fe2+, and SDS. The apparent Km and Vmax with fibrin as the substrate were 3.08 mM and 6.7 nmol/min, respectively. The results suggested that membrane filtration is a useful method for purification of recombinant NK from B. subtilis R0H1. Therefore, application of membrane system is proposed to purify NK at the pilot scale. In addition, our findings indicated that recombinant NK produced in B. subtilis R0H1 showed high and stable proteolytic activity in slightly alkaline pH and at high temperature. It also exhibited strong fibrinolytic activity again both substrates: fibrinogen and fibrin.
54062Purpose, meaning, and passion : HBR emotional intelligence series
This collection of articles includes "Finding Meaning at Work, Even When Your Job Is Dull," by Morten Hansen and Dacher Keltner; "What to Do When Your Heart Isn't in Your Work Anymore," by Andy Molinsky; "You Don’t Find Your Purpose--You Build It," by John Coleman; "How to Find Meaning in a Job That Isn't Your True Calling," by Emily Esfahani Smith; "You're Never Done Finding Purpose at Work," by Dan Pontefract; "From Purpose to Impact," by Nick Craig and Scott A. Snook; "Five Questions to Help Your Employees Find Their Inner Purpose," by Kristi Hedges; "How to Make Work More Meaningful for Your Team," by Lewis Garrad and Tomas Chamorro-Premuzic; "The Power of Small Wins," by Teresa M. Amabile and Steven J. Kramer; and "The Founder of TOMS on Reimagining the Company's Mission," by Blake Mycoskie.
54063Pửt Tày Bắc Cạn
Giới thiệu một loại sinh hoạt văn hóa tín ngưỡng của người Tày ( gọi là Pửt ).
54064Puzzles for programmers and pros
Providing you with the tools to solve several classes of puzzles by handand computer. These include scheduling,strategic, geometric, and probabilistic puzzles. You'll also find a mystery involving codes, bank accounts, and geography that you can solve for the chance to win a prize. The approaches and techniques in this book will help you solve the kind of application puzzles the real world may throw at you.
54065Python for kids
Week 1: Slithering into Python; Week 2: Building Guessing Games; Week 3: Creating Word Games; Week 4: Stepping Up to Object?]Oriented Programming.
54066Python in a nutshell : 2nd ed.
This book offers Python programmers one place to look when they need help remembering or deciphering the syntax of this open source language and its many powerful but scantily documented modules. This comprehensive reference guide makes it easy to look up the most frequently needed information--not just about the Python language itself, but also the most frequently used parts of the standard library and the most important third-party extensions.
54067Python phrasebook
Python Phrasebook gives you the code phrases you need to quickly and effectively complete your programming projects in Python.
54068Python programming for the absolute beginner : 3rd ed.
If you are new to programming with Python and are looking for a solid introduction, this is the book for you. Developed by computer science instructors, books in the "for the absolute beginner" series teach the principles of programming through simple game creation. You will acquire the skills that you need for practical Python programming applications and will learn how these skills can be put to use in real-world scenarios. Throughout the chapters, you will find code samples that illustrate concepts presented. At the end of each chapter, you will find a complete game that demonstrates the key ideas in the chapter, a summary of the chapter, and a set of challenges that tests your newfound knowledge. By the time you finish this book, you'll be well versed in Python and be able to apply the basic programming principles you've learned to the next programming language you tackle.
54069Python Scripting for ArcGIS - First edition (with CD)
1. Introducing python; 2. Geoprocessing in ArcGIS; 3. Using the python window; 4. Learning python language fundamentals; 5. Geoprocessing using python; 6. Exploring spatial data; 7. Manipulating spatial data; 8. Working with geometries; 9. Working with rasters; 10. Map scripting.
54070Qakha Delivery using restful API to build a multi - Platform system of ordering Food and Drinks
Chapter 1: Introduction; Chapter 2: Project overview; Chapter 3: Master plan.