CSDL Bài trích Báo - Tạp chí
chủ đề: Pharmacology
1 Safety investigation of “Thong Ta Yeu Phuong” capsules: Assessment of acute and subchronic toxicity in the experiment / Le Hong Phu, Nguyen Cong Thuc, Dinh Thu Thu Hang // .- 2024 .- Volume 177 E14 - N 04 - May .- P. 98-105 .- 615
“Thong ta yeu phuong” capsules contained natural materials including Atractylodes macrocephala Koidz., Paeonia lactiflora Pall., Pericarpium Citri Reticulatae, and Ligusticum brachylobum Franch. “Thong ta yeu phuong” capsules were intended to support treating gastrointestinal disorders. So far, the safety of this product, however, has not yet beenreported. Thus, this study aimed to investigate the acute and subchronic toxicities of “Thong ta yeu phuong” capsules through oral administration in experimental animals.
2 Acute and sub-chronic toxicities of phatra tricholes capsule in experimental animals / Tran Thanh Tung, Nguyen Thanh Binh, Dang Thi Thu Hien // .- 2024 .- Volume 177 E14 - N 04 - May .- P. 115-123 .- 615
Phatra Tricholes capsule is a multiplant production planned for dyslipidemia patients. Herein, we assessed the potential toxicity of Phatra Tricholes, applying the protocol of acute and sub-chronic oral administration in experimental animal models. According to the WHO guidelines, the acute toxicity study was conducted on Swiss mice.
3 Experimental animal research on the subchronic toxicity of “Dứa Tre Lão Nhà Quê” extract / Pham Thi Van Anh, Vu Viet Hang, Nguyen Thi Thuy, Dau Thuy Duong // .- 2024 .- Volume 177 E14 - N 04 - May .- P. 124-131 .- 615
“Dứa tre Lão nhà quê” extract (DTLNQ extract) was intended to treat several diseases such as dyslipidemia and venous insufficiency. To provide information on the its safety, this study evaluated the subchronic toxicity of DTLNQ extract. Wistar rats were divided into three groups and were given orally for 12 weeks. General conditions and body weight change, hematological and biochemical parameters, and hepatic and renal histological examinations were evaluated in treated rats compared with the control group and baseline.
4 Nasal irritation of Mui Xoang Nhat Nhat spray in experimental animals / Pham Thi Van Anh, Nguyen Thi Thuy, Nguyen Quoc Hoai, Dau Thuy Duong // .- 2024 .- Volume 177 E14 - N 04 - May .- P. 132-138 .- 615
Mui xoang Nhat Nhat (MXNN) spray is a herbal combination of Angelica dahurica, Xanthium strumarium L., Ageratum conyzoides L, which is intended to use as the supportive treatment of stuffy nose, runny nose in some upper respiratory diseases such as rhinitis and sinusitis. This study was carried out to evaluate the nasal mucosa irritation of MXNN spray in experimental animals.
5 Evaluation of acute and subchronic toxicity of “Huyet Phu Truc U Hoan” in experimental animals / Dang Cong Thai, Trinh Hoai Nam, Nguyen Minh Hien, Dang Thi Thu Hien // .- 2024 .- Volume 177 E14 - N 04 - May .- P. 139-148 .- 615
This research was carried out to evaluate the acute and sub-chronic toxicity of ‘‘Huyet phu truc u hoan’’ (HPTUH) in experimental animals. The acute toxicity was defined on Swiss mice by the Litchfield – Wilcoxon’s method and the sub-chronic toxicity on Wistar rat’s hematopoietic function, liver, and kidney functions according to the World Health Organization Guidance.
6 Evaluation of subchronic toxicity of diabetna capsules in experimental animals / Le Hong Oanh, Hoang Minh Chau, Khuat Van Manh, Phuong Thien Thuong, Dau Thuy Duong, Tran Quynh Trang, Pham Thi Van Anh, Dinh Thi Thu Hang, Nguyen Thi Thuy // .- 2024 .- Volume 177 E14 - N 04 - May .- P. 149-157 .- 615
This research aimed to evaluate the subchronic toxicity of Diabetna capsules through oral administration in experimental animals. The subchronic toxicity was studied in Wistar rats with oral doses of 0.72 g/kg/day (equal to recommended human dose) and 2.16 g/kg/day (3 times as high as recommended human dose) in consecutive 12 weeks, following guidance from the World Health Organization and Organisation for Economic Co-operation and Development.
7 In silico study of cytochrome P450 alleles and phenotypic distribution in Vietnamese population / Pham Ngoc Ha, Nguyen Phan Tuan, Trinh Thi Xuan, Truong Nam Hai, Tran Dang Hung, Nguyen Cuong // Công nghệ sinh học - Vietnam Journal of Biotechnology .- 2022 .- Vol 20 - N2 .- P. 197-212 .- 615
Cytochrome P450 enzymes play an important role in phase I drug metabolism, accounting for approximately 75% of the enzymatic processes. We investigated the allele and phenotypic distributions of five important CYPgenes (CYP2B6, CYP3A5, CYP2C9, CYP2C19, CYP2D6) in the Vietnamese population by using Stargazer and the PharmCAT tool to call star alleles and translating them into phenotypes based on the available dataset of PharmGKB. We compared our computational analysis of the Vietnamese distributions with those of East Asia, Europe, America and other super populations,as well aswith previous experimental research. The allele frequencies and phenotypic distributions of the five important CYPgenes in the Vietnamese population are similar to those in East Asia while significantly different fromother populations. In silico analysis also provided consistent results with previous experimental studies. In addition, the resultant data from our research contributes to the database of genetic variations in pharmacogenetics and constructs the fundamentals for future basic and applied research.
8 The first record of Metacordyceps neogunnii (Metacordyceps, Clavicipitaceae) isolated from larva of Lepidoptera in Vietnam: morphological, phylogenetic characterization and chemical constituent analysis / Lao Duc Thuan, Le Huyen Ai Thuy, Le Cong Hac, Nguyen Hoang Mai, Nguyen Van Giang, Truong Binh Nguyen // Công nghệ sinh học - Vietnam Journal of Biotechnology .- 2022 .- Vol 20 - N2 .- P. 317-327 .- 615
An entomopathogenic fungus, specimen DL0091 parasitized on the larvae of Lepidoptera, was collected from Lang Biang Biosphere Reserve, located in Lam Dong Province, Vietnam. The specimen DL0091 has been analyzed to be contained numerous chemical constituents, especially containing adenosine of 634 mg/Kg and cordycepin of 35.2 mg/Kg. Due to containing many bioactive compounds, DL0091 was promised to be a precious natural source that could be applied in fields of medicine and function food for health care. For classification, based on the morphology analysis, it was identified as Metacordyceps neogunnii (Metacordyceps, Clavicipitaceae) sharing the similar characteristics of M. neogunnii T.C. Wen & K.D. Hyde. Morphology of this species differed from Cordyceps neogunnii (Berk.) Berk., by many characteristics, such as the larger stroma of DL0091 (15–130 mm x 2–6 mm), of asci (550–680 μm × 5–8 μm), etc. Additionally, the combined multi-gene phylogenetic analysis, including ITS, Tef and Rpb1, well supported its systematic position in the clade of M. neogunnii, which was used as traditional herb in China and other Asian countries. In summary, DL0091 was identified as M. neogunnii, containing many bioactive compounds, could be used as the medicinal potential in human healthcare.
9 Optimization of Citrus limon peel extraction, determination of main chemical components and effectiveness in repellency against Aedes mosquito dengue fever vector / Phung Thi Kim Hue, Tran Van Loc, Le Tri Vien, Le Dung Sy, Ho Viet Hieu, Le Thi Nhung, Le Thanh Do, Pham Thi Khoa // Công nghệ sinh học - Vietnam Journal of Biotechnology .- 2022 .- Vol 20 - N2 .- P. 329-338 .- 615
In this study, we collected the discarded Citrus limon peel source in Gia Lai province to utilize as a material to build an optimized extraction process with the following criteria: extraction solvent, solvent/sample ratio, extraction temperature, and extraction time. The results showed a stable extraction process with a maximum extraction efficiency of 4.02%, at 40°C, for 3 hours, with two extraction times using 95% ethanol for solvent. Using GC/MS method, the determined limonene content accounted for 12.2% of the extract. The Citrus limon peel extract exhibited potency against Aedes aegypti (arbovirus vector) at a concentration of 0.01 mL, with protection time of 70 minutes and biting percentage of 0.9%, compared to negative control with statistically significant (P < 0.05). The above results correspond with the most recent publications about the effects of mosquito repellence of certain plant-based essential oils. This study has proven that Citrus limon peel in this locality signifies a promising candidature for future studies regarding its main active compound, limonene, in the control of dengue-transmitting vectors. Therefore, Citrus limon peel extract brings hope to develop new mosquito repellency products in the future.
10 Purification and characterization of recombinant nattokinase from Bacillus subtilis R0H1 / Nguyen Thi Thuy Ngan, Le Tuan, Nguyen Lan Huong // Công nghệ sinh học - Vietnam Journal of Biotechnology .- 2022 .- Vol 20 - N2 .- P. 369-377 .- 615
In this study, recombinant NK from B. subtilis R0H1 was purified using two-step membrane filtration. Results showed 3.2-fold increase in activity and a recovery rate of more than 80%. Molecular weight of NK was approximately 28 kDa and its fibrinolytic degradation capacity was proved according to SDS-PAGE. The optimal pH and temperature of this NK were 8.5 and 55°C, respectively. The enzyme activity was boosted by Mg2+, Ca2+ and obviously inhibited by Co2+, Zn+2, Fe2+, and SDS. The apparent Km and Vmax with fibrin as the substrate were 3.08 mM and 6.7 nmol/min, respectively. The results suggested that membrane filtration is a useful method for purification of recombinant NK from B. subtilis R0H1. Therefore, application of membrane system is proposed to purify NK at the pilot scale. In addition, our findings indicated that recombinant NK produced in B. subtilis R0H1 showed high and stable proteolytic activity in slightly alkaline pH and at high temperature. It also exhibited strong fibrinolytic activity again both substrates: fibrinogen and fibrin.